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Combining Additive and Subtractive Techniques in the Design and Fabrication of Microfluidic Devices

From ALine, Inc.
Laminate construction of microfluidic devices offers a means to incorporate both additive and subtractive manufacturing techniques for the development of multiplexed fluidic devices that have, for example, porous membranes formed-in-place (FIP). Important applications for these hybrid devices includes sample preparation, separations, and containment of samples fed by a single inlet and outlet, such as multiple samples of suspended cells in culture contained in their respective wells. We describe the development and performance of a fluidic card for the NASA Ames GeneSat-1 program for autonomous cell culture experiments in space. The fluidic card was constructed as a multi-layer laminate of acrylic and bio-compatible bonding adhesive, using laser ablation to form the channels with porous membranes placed at the inlet and outlet of each sample well to contain individual samples of E. coli and prevent cross contamination.
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